Crystallization of actophorin, an actin filament-severing protein from Acanthamoeba.

نویسندگان

  • K A Magnus
  • S K Maciver
  • T D Pollard
چکیده

Actophorin is an actin monomer-binding and actin filament-severing protein from Acanthamoeba castellanii. It crystallizes out of polyethylene glycol in a form suitable for high resolution x-ray analysis. The crystals are orthorhombic and have the symmetry of the space group P2(1)2(1)2(1) with lattice constants a = 39.8 +/- 0.5, b = 47.3 +/- 0.6, and c = 69.9 +/- 1.6 A. They diffract to a resolution of at least 2.8 A, and the asymmetric unit contains one actophorin monomer of Mr 15,000.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 263 34  شماره 

صفحات  -

تاریخ انتشار 1988